Antigen

Antigen Class

TFs and others

Antigen

ETV6

Cell type

Cell type Class

Bone

Cell type

A-673

Primary Tissue

Skeletal Muscle

Tissue Diagnosis

Rhabdomyosarcoma

Sample

source_name

human Ewing sarcoma cancer cell

cell line

A673

chip antibody

ETV6, Bethyl, A303-674A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For RNA-seq experiments, total RNA was isolated using TRIzol (Thermo Fisher, 15596026) according to the manufacturer's instructions. For ChIP_seq experiments, lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. was constructed using the Illumina TruSeq RNA library prep kit v2 (Illumina, RS-122-2001) or Illumina TruSeq ChIP library prep kit (Illumina, IP-202-1012) based on the manufacturer's instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

55715708

Reads aligned (%)

76.9

Duplicates removed (%)

25.8

Number of peaks

1418 (qval < 1E-05)

hg19

Number of total reads

55715708

Reads aligned (%)

76.2

Duplicates removed (%)

27.3

Number of peaks

1272 (qval < 1E-05)