Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ETV6

Cell type

Cell type Class
Bone
Cell type
A-673
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
Rhabdomyosarcoma

Attributes by original data submitter

Sample

source_name
human Ewing sarcoma cancer cell
cell line
A673
chip antibody
ETV6, Bethyl, A303-674A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For RNA-seq experiments, total RNA was isolated using TRIzol (Thermo Fisher, 15596026) according to the manufacturer's instructions. For ChIP_seq experiments, lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. was constructed using the Illumina TruSeq RNA library prep kit v2 (Illumina, RS-122-2001) or Illumina TruSeq ChIP library prep kit (Illumina, IP-202-1012) based on the manufacturer's instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
55715708
Reads aligned (%)
76.9
Duplicates removed (%)
25.8
Number of peaks
1418 (qval < 1E-05)

hg19

Number of total reads
55715708
Reads aligned (%)
76.2
Duplicates removed (%)
27.3
Number of peaks
1272 (qval < 1E-05)

Base call quality data from DBCLS SRA